Beschreibung
ROTIPHORESE® Sequencing gel diluent, 50 % urea (w/v,8.3 M), in water, ready-to-use, Density (D) 1,12 g/cm³, Boiling point (bp) 100 °C, Storage temp. +4 to +15 °C
- For preparation of 4-20 % sequencing gels
- Most simple handling of three optimised ready-to-use solutions
- No tedious dissolving of urea
- Prevents acrylamide dusts
- Reproducible, superior quality of gels
- In tried-and-tested ROTIPHORESE® quality
Convenient set containing three ready-made solutions for easy and fast casting of sequencing gels. Solutions are produced in ROTIPHORESE® quality and are perfectly matched - providing superior, reproducible quality and high sequence resolution.In addition, the ROTIPHORESE®Sequencing system allows flexible adaptation to the desired gel variant, so that the amount of urea can be varied in addition to the acrylamide concentration. By using the ROTIPHORESE®Sequencing gel buffer concentrate and ROTIPHORESE®Sequencing gel diluent contained in the set, gels with 50 % urea content are obtained. By adding defined quantities of ROTIPHORESE®10x TBE buffer or water, the urea content can be reduced to up to 40 %.
Handling is most simple: Measure and mix certain amounts of all three components, add APS and TEMED, and cast the gel. We recommend using the Carl ROTH sequencing electrophoresis chamber (Art. no. AE28).
Ready-to-use, aqueous 50 % urea stock solution. For the preparation of polyacrylamide sequencing gels with 40-50 % urea (6,7-8,3 M).
Also part of the ROTIPHORESE®DNA sequencing system (Art. No. A431.1).