Beschreibung
anti-bèta-actine, kloon AC-15,, monoklonaal muis-antilichaam, 100 µg/ml, affiniteit-gezuiverd (geproduceerd door PROGEN), dichtheid (D) ~1 g/cm³, kookpunt (bp) ~100 °C, bewaartemp. +4 °C
Dit antilichaamportfolio bevat een breed scala aan weefsel- en tumorspecifieke primaire antilichamen voor fundamenteel onderzoek, productontwikkeling en de analyse van uiteenlopende biologische vraagstukken. De monoklonale antilichamen zijn uitermate geschikt voor alle cel- of weefselbiologische studies en voor de analyse van predictieve markers in onderzoek.
- Hoge epitoop-affiniteit
- Zeer specifieke detectie van het doelwit-eiwit
- Niet-geconjugeerd
- Geschikt voor bijv. western blot, immunoprecipitatie, ELISA & immunofluorescentie
Bèta-Actine (42 kDa) is een sterk geconserveerd eiwit dat kan polymeriseren tot filamenten. Het is betrokken bij celmotiliteit, structuur en integriteit. Bèta-Actine wordt veel gebruikt als loading control voor western blotting of als housekeeping-genstandaard bij qPCR.
Immunohistochemie (IHC) - paraffine: 1:50-1:500 (0,2-2 μg/ml; magnetronbehandeling aanbevolen) Western Blot (WB): 1:5.000 (20 ng/ml)
| Toepassing | IHC WB |
| Gastheersoort | muis |
| Klonale status | monoklonaal |
| Reactiviteit antilichaam | humaan |
| Zuivering | Affiniteitschromatografie |
| Conjugaat | niet-geconjugeerd |
| Concentratie | 100 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | P62739 (rund), P08023 (kip), P62736 (humaan), P62737 (muis), P62738 (rat) |
| Kloon | AC-15 |
| Doelwit | Bèta-Actine |
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Questions about anti-bèta-actine, kloon AC-15, muis monoklonaal, 0,2 ml, kunststof
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anti-bèta-actine, kloon AC-15, muis monoklonaal, 0,2 ml, kunststof is the article on this page. Luminix Health distributes PROGEN for laboratory use. It sits in Primary antibodies, inside Antibodies & ELISA kits, in Mikrobiologie.
A primary antibody binds the target. Host species, clonality, clone name if monoclonal, the antigen, and the applications it was shown in, such as Western blot or immunohistochemistry, are the specification. A pretty band in the wrong application is not a promise.
An antibody binds one target. A primary antibody binds the molecule of interest. A secondary antibody binds the primary and usually carries the signal, an enzyme or a dye. An ELISA kit is that binding arranged in a well so the amount of target becomes a colour. The host species, the clone, the antigen and the stated application are the specification. A name alone is not. The literature is on PubMed. Sequences of the targets are on NCBI Nucleotide. Reference material is catalogued by ATCC.
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