anti-DDDDK-tag mouse, monoclonal, AP1501, after dissolv. in 100 µl PBS

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anti-DDDDK-tag mouse, monoclonal, AP₁₅₀₁, after dissolv. in 100 µl PBS, 250 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) 100 °C, Storage temp. +4 °C

Specific primary antibodies that recognise the most commonly used protein tags. The antibodies can be excellently used in cell or tissue biology studies, protein-protein interaction assays, post-translational modifications, expression dynamics or localisation studies, up to the production of analytical kits in laboratory or large scale.

Available for most commonly used protein tags (His, DDDDK, GST, HA, myc, GFP & Strep)
High affinity to the respective protein tag
Robust & highly specific detection of the fusion protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

The monoclonal AP1501 antibody recognizes the amino acid sequence DDDDK (Flag). The DDDDK-tag is commonly added to recombinant proteins and can be used for detection or purification of the tagged protein.

Application

Immunocytochemistry (ICC): assay dependentImmunoprecipitation (IP): assay dependentWestern Blot (WB): 1:50,000-1:100,000 (0.005-0.0025 μg/ml)

Western blot analysis of E. coli lysate containing DDDDK-tagged protein with anti-DDDDK-tag antibody. Western blot analysis was performed on 80 ng, 40 ng or 10 ng of E. coli lysate containing DDDDK-tagged protein. Cells were lysed with SDS sample buffer. The PVDF membrane was blocked with 5 % dry milk in PBST for 1 h at RT. The primary antibody anti-DDDDK-tag mouse monoclonal, AP1501 (Cat. No. 910DDKL) was diluted in blocking buffer (antibody concentration 0.005 μg/ml) and incubated for 1 h at RT. The secondary antibody goat anti-mouse IgG polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IP WB
Host species	mouse
Clonality	monoclonal
Purification	Affinity chromatography
Conjugate	uncojugated
Isotype	IgG2b
Clone	AP1501
Target	DDDDK (FLAG)-Tag

Technical Documentation (PDF)

anti-DDDDK-tag mouse, monoclonal, AP1501, after dissolv. in 100 µl PBS, 100 µl

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