Beschreibung
anti-Desmoplakin 1/2 mouse, monoclonal, DP 1 + 2-2.15, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C
This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.
- High epitope affinity
- Highly specific detection of the target protein
- Unconjugated
- Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence
DP1 + 2-2.15 shows distinct punctate membrane staining of different epithelia. Tumors specifically detected: primary and metastatic carcinoma and meningioma. Polypeptides reacting: desmoplakin 1 and 2 (Mr 250,000 and 215,000). The epitope recognized by clone DP1 + 2-2.15 is mapped to the N-terminus of the rod domain (Bornslaeger, E. A., 1996). Bornslaeger, E. A. Breaking the connection: displacement of the desmosomal plaque protein desmoplakin from cell-cell interfaces disrupts anchorage of intermediate filament bundles and alters intercellular junction assembly. J. Cell Biol. 134, 9851001 (1996).
Immunocytochemistry (ICC): Assay dependentImmunohistochemistry (IHC) - frozen: 1:10-1:50 (1-5 μg/ml) Immunohistochemistry (IHC) - paraffin: 1:10-1:50 (1-5 μg/ml, microwave treatment recommended) Western Blot (WB): assay dependent
| Application | ICC/IF IHC WB |
| Host species | mouse |
| Clonality | monoclonal |
| Reactivity antibody | rat cattle |
| Purification | Affinity chromatography |
| Conjugate | uncojugated |
| Concentration | 50 µg/ml |
| Isotype | IgG1 |
| Uniprot ID AK | A0A3Q1MR22 (bovine), E1BWI0 (chicken), P15924 (human), E9Q557 (mouse), Q8VBY1 (rat) |
| Clone | DP1 + 2-2.15 |
| Target | bovine desmoplakin 1 + 2 |