Beschreibung
anti-Strep-tag muis, monoklonaal, , C23.21, na oplossen in 250 µl PBS, 100 µg/ml, affiniteit-gezuiverd (geproduceerd door PROGEN), dichtheid (D) ~1 g/cm³, kookpunt (bp) 100 °C, opslagtemp. +4 °C
Specifieke primaire antilichamen die de meest gebruikte eiwittags herkennen. De antilichamen zijn uitstekend bruikbaar voor cel- of weefselbiologisch onderzoek, eiwit-eiwit-interactie-assays, post-translationele modificaties, expressiedynamiek of localisatieonderzoek, tot aan de productie van analytische kits op laboratorium- of grootschalig niveau.
- Beschikbaar voor de meest gebruikte eiwittags (His, DDDDK, GST, HA, myc, GFP & Strep)
- Hoge affiniteit voor de betreffende eiwittag
- Robuuste & zeer specifieke detectie van het fusie-eiwit
- Niet-geconjugeerd
- Geschikt voor bijv. western blot, immunoprecipitatie, ELISA & immunofluorescentie
Het monoklonale C23.21-antilichaam herkent Strep II. Een Strep-tag wordt doorgaans toegevoegd aan recombinante eiwitten en kan worden gebruikt voor detectie of zuivering van het getagde eiwit.
Western Blot (WB): 1:5.000-1:10.000 (0,02-0,01 μg/ml)
| Toepassing | WB |
| Gastheersoort | muis |
| Klonale status | monoklonaal |
| Zuivering | Affiniteitschromatografie |
| Conjugaat | niet-geconjugeerd |
| Isotype | IgG1 |
| Kloon | C23.21 |
| Target | Strep-tag |
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A primary antibody binds the target. Host species, clonality, clone name if monoclonal, the antigen, and the applications it was shown in, such as Western blot or immunohistochemistry, are the specification. A pretty band in the wrong application is not a promise.
An antibody binds one target. A primary antibody binds the molecule of interest. A secondary antibody binds the primary and usually carries the signal, an enzyme or a dye. An ELISA kit is that binding arranged in a well so the amount of target becomes a colour. The host species, the clone, the antigen and the stated application are the specification. A name alone is not. The literature is on PubMed. Sequences of the targets are on NCBI Nucleotide. Reference material is catalogued by ATCC.
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