Beschreibung
BrightGreen DNA dye, for electrophoresis, ready-to-use, 11x concentrated, Density (D) 1 g/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C
This is the DNA dye BrightGreen I, a staining reagent for detection of double stranded nucleic acid in agarose and polyacrylamide gels. The dye is added directly to the DNA/loading buffer mixture and then loaded onto the gel.BrightGreen I bounds to the minor groove of DNA and intercalates into the DNA. Upon excitation it emits a bright green fluorescence. The sensitivity is up to 0.01 ng per band and is therefore 20 times higher than ethidium bromide.BrightGreen I has been developed specifically for gentle DNA analysis. DNA integrity is not influenced; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligations, PCR, transformation, transfection etc. without interfering effects.
- Recommended for dsDNA
- Direct addition to DNA sample
- For excitation with UV light and blue light
- Non-toxic alternative for ethidium bromide
- Sensitivity (0,01 ng/band)
BrightGreen DNA dye can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1). The gels may be documented by all usual broadband ethidium bromide- or BrightGreen foto filters.
Excitation maximum (bound to DNA): approx. 254 nm and 495 nmEmission maximum (bound to DNA): 521 nm
| Max. excitation (DNA-bound) | 254 nm495 nm |
| Amount of traces | ca. 2000/ml |
| Emissions max. (DNA-bound) | 521 nm |
| Sensitivity | 0,01 ng/band |
| ROTH for Future Score - sustainability criteria | • Product features You can find further information on our website - Sustainability criteria at Carl ROTH. |
| ROTH for Future | yes |