Beschreibung
Gelfiltratiemedia, ROTI®Dex-50 Medium, Opslagtemp. +2 tot +25 °C, CAS-nr. 9048-71-9
ROTI®Dex Medium is een bolvormig, poreus gelfiltratiemedium bestaande uit met epichloorhydrine gecrosslinkte dextran.
Bij gelfiltratie, ook wel gelpermeatiechromatografie genoemd, worden moleculen gescheiden op basis van hun grootte in oplossing. Kleinere moleculen passeren de kolom veel langzamer dan grotere moleculen. In tegenstelling tot andere chromatografiemethoden treden hier geen interacties op tussen de analyt en de stationaire fase van de kolom. Dit biedt de ideale basis voor de opzuivering en analyse van intacte eiwitten.
ROTI®Dex maakt een vorm van groepsscheiding mogelijk voor de afscheiding van ongewenste, laagmoleculaire factoren zoals onzuiverheden, zouten, hulpstoffen, markers, celresten, enz. uit eiwit- of nucleïnezuuroplossingen.
- Monstervoorbereiding in slechts enkele minuten
- Ontzouten, verwijdering van onzuiverheden en bufferextractie in één stap
- Geen interactie tussen analyt en medium
- Hoge selectiviteit, hoge resolutie en hoge opbrengst (> 95%)
- Ook geschikt voor kleine monstervolumes
- Hoge chemische stabiliteit en daarom compatibel met vele organische en andere oplosmiddelen
ROTI®Dex-50 medium is uitstekend geschikt voor de scheiding van eiwitten >25 kDa en nucleïnezuren >20 bp. Opgezuiverde biomoleculen worden niet significant verdund bij verwerking met ROTI®Dex-50 medium.
Gedetailleerde informatie over de toepassing vindt u in onze Technische Informatiebrochure en in onze gebruiksaanwijzing.
| Matrix | Gecrosslinkte dextran |
| MWCO | 25 kDa20 bp |
| Deeltjesgrootte | Droog: 50-150 µmNatte: 100-300 µm |
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