Beschreibung
Hyaluronidase, ovien, gevriesdroogd, Molaire massa (M) 55 000 g/mol, Opslagtemp. -20 °C, CAS-nr. 37326-33-3, EG-nr. 253-464-3
Hyaluronidase klieft willekeurig endo-N-acetylhexosamine-bindingen in hyaluronzuur, chondroïtine en chondroïtinesulfaten.
Aangezien hyaluronzuur en chondroïtinesulfaat vaak voorkomen in bindweefsel, wordt hyaluronidase in combinatie met collagenase meestal gebruikt voor celdissociatie. De enzymen breken de extracellulaire matrix af, wat leidt tot het losmaken van cellen uit dierlijk weefsel of kweekoppervlakken.
Eén eenheid is gedefinieerd als de hoeveelheid enzym in een hyaluronzuur-albumine-mengsel die dezelfde troebelheidsreductie teweegbrengt als 1,0 eenheid van het internationale standaardpreparaat.
| Aanvullende info | Bron: Ovien |
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Hyaluronidase, schaap, gevriesdroogd, 50 mg, glas is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Enzymes & substrates, inside Biochemicals & reagents, in Mikrobiologie.
An enzyme is sold by activity, in units defined by a stated reaction, not only by milligrams. Source and specific activity are the specification. A substrate is the molecule that enzyme converts, often a chromogenic one so the reaction can be seen. Units from two suppliers are comparable only when the definition matches.
A biochemical is a molecule of life, sold as a reagent: an amino acid, a sugar, a nucleotide, a lipid, an enzyme, an antibiotic. The difference from a bulk chemical is that the use is a biological one, and the impurities that matter are the ones that change that use. An enzyme is specified by activity, not only by mass. An antibiotic is specified by potency. Papers are on PubMed. Structures are on PubChem. Strains and lines that consume these reagents are at ATCC.
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