Beschreibung
RIPA-buffer, 1x conc., klaar voor gebruik, voor eiwitextractie, dichtheid (D) 1,052 g/cm³, kookpunt (kp) ~100 °C, opslagtemp. +2 tot +8 °C
De radio-immunoprecipitatiebuffer, ook bekend als RIPA-buffer, is een van de meest betrouwbare cellysebuffers voor snelle en efficiënte lyse van zoogdiercellen uit adhesie- of suspensieculturen.
Het maakt de extractie en solubilisatie van eiwitten uit het membraan, de celkern en het cytoplasma mogelijk.
- Extractie van eiwitten uit talrijke celtypes
- Efficiënte cellyse en solubilisatie van eiwitten
- Eiwitdegradatie wordt voorkomen
- Geen interferentie met de meeste antilichamen en eiwitantigenen
- Minimaliseert niet-specifieke eiwitbindingsinteracties
- Compatibel met vele toepassingen, waaronder reporter-assays, eiwitassays, immunoassays en eiwitzuiveringen
| Toepassing | 1 ml RIPA-buffer is voldoende voor de lyse van 0,5 tot 5 x 107cellen (75 cm2 cellaag). |
| Gebruiksaanwijzing, toevoeging | Protease- en fosfataseremmers kunnen naar behoefte aan de lysebuffer worden toegevoegd. |
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RIPA-buffer, 1x conc., gebruiksklaar, voor eiwitextractie, 100 ml, kunststof is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Ready-to-use culture media (plates, tubes, bottles), inside Culture media & microbiology, in Mikrobiologie.
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