Beschreibung
ROTI®Load DNAstain 1 Green,, 6x DNA gel loading buffer, ready-to-use, DNase-free, Density (D) ~1,12 g/cm³, Storage temp. -20 °C
Loading bufer with the fluorescent dye SYBR® Green I for staining of double stranded nucleic acid in agarose and polyacrylamide gels.
- Alternative for ethidium bromide, non-toxic, non-mutagen
- 20 times more sensitive than ethidium bromide, up to 0.01 ng nucleic acid per band
- For use with common broad-band ethidium bromide photo filters
- Excitation possible via UV light (254 nm) and blue light (495 nm)
- Compatible with all usual down-stream applications.
ROTI®Load DNAstain SYBR® Green is easy to use and reduces significantly the time required for gel analysis. The contained fluorescent dye SYBR® Green I detects up to 0.01 ng per band, and is, therefore, 20 times more sensitive than ethidium bromide. DNA integrity is not influenced by SYBR® Green I; hence, gel eluted DNA may directly be applied to standard down-stream applications, like, e.g., ligation, PCR, transformation, transfection etc. without interfering effects.SYBR® Green I bounds to the minor groove of DNA and intercalates into the DNA. It can be excited by UV- and blue light, making it fully compatible with standard UV-transilluminators as well as with modern blue-light illumination (see also ROTIPHORESE®PROfessional runVIEW, Art. No. 4849.1).When excited, nucleic acid-bound SYBR® Green I emits a brightly coloured green fluorescence (521 nm) that may be documented by all usual broadband ethidium bromide- or SYBR® Green foto filters.The figure depicts 50 ng DNA per band, or 75 ng (1000 bp and 2.5 kb), respectively. Each lane has been loaded and concurrently stained using 1 µl ROTI®Load DNAstain SYBR® Green.
Excitation maximum (bound to DNA): 254 nm and 495 nmEmission maximum (bound to DNA): 521 nm
In order to cover the main size range needed in gel electrophoresis, ROTI®Load DNAstain 1 Green contains bromophenol blue and xylene cyanol.
Glycerin is the standard reagent for increasing sample density.
Recommended for staining of dsDNA- and dsRNA-fragments of over 500 bp.
| Max. excitation (DNA-bound) | ~320 nm470-500 nm |
| Amount of traces | ca. 1000/ml |
| Emissions max. (DNA-bound) | 525 nm |
| Dye | Bromphenol blue, xylene cyanole, BrightGreen |
| Incl. DNA staining | yes |
| Sensitivity | 0,01 ng/band |
| Use | Simultaneous gel staining, bigger fragments |