Beschreibung
ROTI®Prep Gel & PCR, 10 pcs, pour la biologie moléculaire, Density (D) 1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep Gel & PCR is designed to provide the greatest flexibility in the purification of isolated DNA fragments. The kit is used for the purification of DNA from PCR reactions as well as for the purification from gel pieces.The purification from PCR and sequencing reactions is based on a new two-step process in which the DNA is first bound to the column membrane using the Binding Buffer and then eluted in the second step.In the purification from agarose gel pieces, the gel piece is first dissolved and the DNA is then washed in several washing and centrifugation steps before it can be eluted.
- Great flexibility through dual use
- Preparation in the well-established mini-column system
- Fast, easy and reliable
- Extraction time: approx. 3 min (sequencing and PCR reactions), approx. 20 min (gel extraction)
- TAE and TBE agarose gels (up to 300 mg)
- PCR reactions (up to 50 µl)
- Sequencing reactions (up to 50 µl)
Gel Solubilizer, Binding Enhancer, Binding Buffer, Washing Solution WSL (conc.), Elution Buffer EB, centrifugation columns, collection tubes. Elution tubes, detailed instructions.Ethanol 99.8 % (p.a., Art. no. 9065) is not included in the kit and should be ordered separately if required.
| Basic material | PCR/sequencing reactions, TAE and TBE agarose gels |
| Quantity of material | < 50 μl reaction mixture, < 300 mg agarose gel |
| Preparation time | approx. 3 minutes (sequencing and PCR reactions), approx. 20 minutes (gel extraction) |
| Instructions for use, addition | Please note the different protocols for purification from different starting materials. |
| Automatic use | no |
| Elution volume | 30-50 µl |
| Typical purity (A260/A280) | 1.7-2.0 |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)