anti-Glial Fibrillary Acidic Protein, mouse monoclonal, GF 12.24, 0.2

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anti-Glial Fibrillary Acidic Protein, mouse monoclonal, GF 12.24, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

High epitope affinity
Highly specific detection of the target protein
Unconjugated
Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

GF 12.24 represents an excellent marker for cell typing. Suitable for prenatal diagnosis of neural tube defects. Polypeptide reacting: Mr 50,000 glial filament protein GFAP (Glial Fibrillary Acidic Protein, Glial Filament Protein). Tumors specifically reacting: astrocytomas, gangliomas, medulloblastomas, mixed gliomas, certain ependymomas, certain teratomas. Reactivity on cultured cell lines: human U 333CG/343MG

Application

Immunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:100-1:200 (250-500 ng/ml) Immunohistochemistry (IHC) - paraffin: 1:100-1:200 (250-500 ng/ml; microwave treatment recommended) Western Blot (WB): assay dependent

IHC analysis of mous brain using anti-GFAP antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24 (Cat. No. 690011) was diluted in PBS (antibody concentration 250 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).

IHC analysis of rat brain using anti-GFAP antibody. IHC was performed on formalin fixed paraffin embedded sections. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. After preparation the tissue was blocked with normal serum for 20 min at RT. The primary antibody anti-Glial Fibrillary Acidic Protein mouse monoclonal, GF 12.24 (Cat. No. 690011) was diluted in PBS (antibody concentration 250 ng/ml) and incubated at 4°C over-night. The secondary antibody ImmPRESS HRP anti-mouse IgG was incubated for 20 min at RT. Slides were incubated with DAB solution until a brown staining is visable and with Haemalaun for a few minutes. The 20x picture was acquired using microscopy (courtesy of J. Hess, University Hospital Heidelberg).

Not a medical device / Not an IVD product

Technical Information

Application	ICC/IF IHC WB
Host species	mouse
Clonality	monoclonal
Reactivity antibody	rat cattle
Purification	Affinity chromatography
Conjugate	uncojugated
Concentration	50 µg/ml
Isotype	IgG2a
Uniprot ID AK	Q28115 (bovine),P14136 (human),P03995 (mouse), P47819 (rat)
Clone	GF 12.24
Target	intermediate filament cytoskeleton from cultured human glioma cells

Technical Documentation (PDF)

anti-Glial Fibrillary Acidic Protein, mouse monoclonal, GF 12.24, 0.2 ml

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