Beschreibung
HPLC Column NUCLEODUR®, PFP, 5µm, 250x3mm
Material: stainless steel.
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This hydrophobic phase with pentafluorophenyl-propyl modification is based on high purity NUCLEODUR® silica gel and gives an alternative selectivity in comparison to classical C18 modifications. Separation mechanism based on four retention mechanisms - polar interactions (H bonds), dipole-dipole interactions, π-π interactions and hydrophobic interactions. Suitable for LC/MS due to low bleeding characteristics.
Delivery incl. 1x analytical HPLC column, instruction leaflet, certificate of analysis.
Technical Information
| Particle size | 5 µm |
| Pore size | 110 Å |
| L x Ø internal (column) | 250 x 3 mm |
| Column volume | 1.75 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | PFP |
| Surface chemistry | Pentafluorophenylpropyl (PFP) modification |
| USP listing | L43 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | polar (H-bond), dipole-dipole, π-π and hydrophobic |
| Pressure stability | 600 bar |
| pH stability | 1,0-9,0 |
| Recommended application(s) | Antibiotics Aromatic and unsaturated compounds Basic compounds Suitable for LC-MS Halogenated compounds Isomers Pharmaceutical products |
| Application Database (Macherey-Nagel) | HPLC |