Beschreibung
HPLC Column NUCLEODUR®, HILIC, 3µm, 150x3mm
Material: stainless steel.
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Separation science is always looking for new and effective strategies to accomplish the tasks of modern analytics. Especially for polar compounds reversed phase HPLC - the most common analytical method - is often limited. Here, hydrophilic stationary phases provide an additional tool for the separation of polar analytes in HPLC. HILIC is NP chromatography of polar and ionic compounds under RP conditions. The zwitterionic HILIC phase is based on high purity NUCLEODUR® silica gel.
Delivery incl. 1x analytical HPLC column, instruction leaflet, certificate of analysis.
Technical Information
| Particle size | 3 µm |
| Pore size | 110 Å |
| L x Ø internal (column) | 150 x 3 mm |
| Column volume | 1.05 ml |
| Endcapped | no |
| Eluent in column | Acetonitrile - Water |
| Phase | HILIC |
| Surface chemistry | Ammonium – sulfonic acid (zwitterionic) modification |
| USP listing | Not available or proprietary |
| Separation principle | Hydrophilic Interaction Chromatography (HILIC) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | ionic / hydrophilic and electrostatic |
| Pressure stability | 600 bar |
| pH stability | 2,0-8,5 |
| Recommended application(s) | Amino acids HILIC applications Highly polar analytes Hydrophilic compounds Nucleosides Oligonucleotides Organic polar acids and bases |