Beschreibung
ROTI®Fect, ready-to-use, CELLPURE®, for transfection, Density (D) ~1 g/cm³, Storage temp. +4 °C
ROTI®Fect is a liposome-based transfection reagent consisting of a mixture of polycationic and neutral lipids. Compared to monocationic lipid formulations, ROTI®Fect has a higher efficiency with lower levels being required. Its excellent reproducibility in combination with its high level of efficiency reduced optimisation work to a minimum. Because of its low toxicity, a wide range of cell lines can be transfected efficiently.An efficient triple-cotransfection using ROTI®Fect is described in Ufartes, Schlüter, Kaulfuss and Seipp (2005) Biospektrum 4: 464-5.
- Highly efficient reproduction of DNA in mammal cells
- Excellent reproducibility
- High and wide operating plateau
- No inhibition through serum
- Low cell toxicity
- Successful with different cell lines
- Excellent transfection results
siRNA: CEM, COS-7, ES-D3,HeLa, HL60, LNCaP, OVCAR3, PC3, SW620Primary cells: pAML, pHDF, pMEF, pRHC, pSMCCell lines: 143B, 293-T, A-293, A-431, AM-C6SC8, BHK, BV-2, C2C12, C6, CHO, CHO-DHFR, COS-1, COS-7, CRFK, Colo-205, DU-145, ECV-304, EMC, F98 (glioma), GD25-ß1, HBL 100, HCT-116, HCT-15, HEK293, HeLa, HeLa-S3, Hep-3B, Hep-G2, HOSE, HSG, HT-22, HT-29, HUVEC, JURKAT, LLC-PK1, LS174T, LoVo, MCF-7, MDCK, MeWo, MV3, NIH-3T3, NS20Y, OK, P815, PC3, PT-11, Rcho-1, SAOS, SH-SY5Y, SK-MEL-28, SKOV-3, SM10, SW-480, THP-1, tsA201, U87 (glioma), Vero 76, et al.
Approx. 400 assays per ml in 6 wells. Dependent on the cell line used.