Beschreibung
ROTI®Garose-Glu/GST Beads, for biochemistry, Density (D) 0,85 to 0,95 g/cm³, Flash point (flp) >50 °C, Boiling point (bp) >80 °C, Storage temp. +4 °C, UN-Nr. 1170
Glutathione coupled agarose beads for isolation of glutathione-S-transferase (GST) or GST fusion proteins.ROTI®Garose-Glu/GST Beads offer the perfect solution for your specific application of low flow column chromatography or protein isolation in batch mode, under medium pressure (MPLC, FPLC), if rapid flow rates are desired or if big samples volumes have to be purified.
- Rapid, mild and highly specific purification
- Suitable for isolation of GST-peptides and protein complexes of all sizes
- High binding capacity
- Easy elution and regeneration
Efficient and highly specific isolation of GST-tagged proteins via affinity chromatography is based on the affinity of the glutathion-S-transferase to it’s substrate glutathione. Merely no unspecific binding of proteins without GST-tag takes place, even during purification from raw extracts, while the biological activity of the purified proteins is preserved by chromatography conditions.
The matrix of ROTI®Garose-Glu/GST Beads consists of beaded 4 % agarose without crosslinking, coupled with glutathione. Elution is carried out via a mild Tris/glutathione buffer, enabling even recovery of native protein complexes. ROTI®Garose-Glu/GST Beads may be regenerated, making them very cost-effective.
Glutathione coupled agarose beads for affinity chromatography.75 % bead suspension in 20 % ethanol.
- One-step isolation of GST-tagged proteins from total lysates
- For batch mode, gravity flow, MPLC und FPLC
- Suitable for big matrix volumes
Recommended for isolation of glutathione-S-transferase (GST) or GST fusion proteins in batch mode or via columns. Suitable for low to medium pressure, FPLC, or if GST-tag proteins shall be isolated either in particularly short time or in big amounts.
The matrix is easy to pack and results in very evenly packed columns.
| Instructions for use, addition | Bead suspension, binding capacity ≥8 mgl/ml packed matrix |
Compliance & Safety Information
Warnung
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)