Description
3,3'-Diaminobenzidine tetrahydrochloride, min. 98 %, p.a., Molar mass (M) 360,10 g/mol, Flash point (flp) 282,7 °C, Boiling point (bp) 481,7 °C, Storage temp. +4 °C, CAS No. 7411-49-6, EC No. 231-018-9, Empirical formula C12H14N4 · 4 HCl
Popular substrate of peroxidases. DAB is used for biochemical and histological detection of endogenous peroxidases like catalase and cytochome oxidase. Additionally, it is the most common substrate for horseradish peroxidase (HRP) in histochemical and cytological assays. DAB is oxidised by HRP, forming a brown precipitate, which is unsoluble in aqueous and organic solvents. This precipitate can be detected in visible light and does not bleach during long-term storage.
Working concentration: 0.05-0.1 % (0.5-1 mg/ml) DAB in buffer (PBS, TBS, pH 7.0-7.6) containing 0.01 % hydrogen peroxide. Always use freshly prepared working solution. For oxidation high concentrations of fresh hydrogen peroxide are needed.
| Appearance | fast weißes bis braunes Pulver |
| Application | Western, in situ, Histochemistry |
| Enzyme | Horseradish Peroxidase |
| Detection | Colour (brown) |
| Signal product | Precipitate (Blot) |
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)