anti-Keratin K7 mouse, monoclonal, Ks7.18, 0.2 ml, plastic - Luminix Health
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anti-Keratin K7 mouse, monoclonal, Ks7.18, 0.2 ml, plastic - Luminix Health

anti-Keratin K7 mouse, monoclonal, Ks7.18, 0.2 ml, plastic

SKU: 1NNN.1
Volume: 0.2 ml
Value Packs: 1 piece
Price:
€109,15

Description

anti-Keratin K7 mouse, monoclonal, Ks7.18, 50 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

Ks7.18 represents an excellent marker for the discrimination of specific subtypes of adenocarcinoma: e.g. adenocarcinoma of pancreas, bile duct carcinoma and transitional carcinoma of bladder are stained, whereas hepatocellular and prostate carcinomas are negative. Detects specific subtypes of adenocarcinomas: adenocarcimona of pancreas, gallbladder, lung, cervix; cholangio carcinoma of liver; ductal and lobular carcinoma of breast; carcinomas of ovary ; transitional cell carcinoma of bladder; mesothelioma; negative with most cases of hepatocellular carcinoma. In colorectal carcinoma early stages are reported to be negative but advanced stages of tumor development are positive for keratin K7 expression. Occasionally, staining of blood vessel walls, particularly of endothelial cells may be observed. Reacts with Mr 54,000 polypeptide (keratin K7; formerly also designated cytokeratin 7) of human glandular epithelia. Reactivity on cultured cell lines: HeLa, RT 112, T24, BT-20, CAMA-1, Detroit 562, (MCF-7 and HT-29 are negative).

Application

Immunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:50-1:250 (0.2-1 μg/ml)Immunohistochemistry (IHC) - paraffin: 1:50-1:250 (0.2-1 μg/ml, protease treatment and/or microwave treatment recommended)Western Blot (WB): 1:1,000-1:2,000 (25-50 ng/ml)

Human thyroid carcinoma (courtesy of J.Heß, University Hospital Heidelberg)
Western blot analysis of RT-4 lysate with anti-Keratin K7 antibody. Western blot analysis was performed on 20 μg RT-4 lysate. Cells were lysed with RIPA buffer. The PVDF membrane was blocked with 5% dry milk in PBST (PBS + 0.1% Tween 20) for 1 h at RT. The primary antibody anti-Keratin K7 mouse monoclonal, Ks7.18 (Cat. No. 690025) was diluted in blocking buffer (antibody concentration 50 ng/ml) and incubated for 1 h at RT. The secondary antibody anti-mouse IgG goat polyclonal, HRP conjugate was also diluted in blocking buffer (antibody concentration 0.2 μg/ml) and incubated for 1 h at RT. The bands were visualized by chemiluminescent detection using Pierce™ ECL Western Blotting Substrate.
Human kidney (courtesy of J. Heß, University Hospital Heidelberg)
Not a medical device / Not an IVD product
Technical Information
Application ICC/IF IHC WB 
Host species mouse 
Clonality monoclonal 
Reactivity antibody cattle pig human sheep 
Purification Affinity chromatography 
Conjugate uncojugated 
Concentration 50 µg/ml 
Isotype IgG1 
Uniprot ID AK Q29S21 (bovine), P08729 (human), A0A287ASI0 (Pig), H0VIA2 (guinea pig), A0A6P7DW90 (sheep) 
Clone Ks7.18 
Target Keratin K7 

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