Description
HPLC column NUCLEOSHELL®, Biphenyl, 2.7µm, 50x4.6mm
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Hydrophobic phase with alternative selectivity compared to classical C18 modifications. The separation principle based on two retention mechanisms - π-π interactions and hydrophobic interactions. This provides better retention of aromatic and unsaturated substances. This stationary HPLC phase is based on core-shell technology silica gel particles and shows excellent performance under highly aqueous conditions. The special phase modification of NUCLEOSHELL® Biphenyl with iso-butyl sidechains leads to low bleeding characteristics even at very acidic pH values compared to competitor columns. Due to these iso-butyl sidechains and multi-endcapping procedures no phase collapse occurs and stability in 100 % aqueous mobile phase is ensured.
| Particle size | 2.7 µm |
| Pore size | 90 Å |
| L x Ø internal (column) | 50 x 4.6 mm |
| Column volume | 0.83 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | Biphenyl |
| Surface chemistry | Biphenylpropyl modification |
| USP listing | L11 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Core-shell, superficially porous particles (SPP) |
| Specific surface according to BET | 130 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | π-π and hydrophobic |
| Pressure stability | 600 bar |
| pH stability | 1,5-8,5 |
| Recommended application(s) | Antibiotics Aromatic and unsaturated compounds DNPH Aldehydes hormones Mycotoxins Pesticides Pharmaceutical products |
| Application Database (Macherey-Nagel) | HPLC |