ROTI®Blue quick, 1x solution, ready-to-use, 250 ml, glass

SKU: 4829.1

Volume: 250 ml
Value Packs: 1 piece
Price:
Sale price€112,90

Description

ROTI®Blue quick, 1x solution, ready-to-use, staining solution for protein gels, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C, UN-Nr. 1760

ROTI®Blue quick provides a new, very fast staining method for proteins in polyacrylamide gels. Due to the modern formulation, the dye binds highly specifically to proteins, but not to the gel matrix, hence eliminating the need to destain the gel. First bands are clearly visible within 3 minutes!

  • Particularly fast, specific staining
  • First bands are visible within 3 mins.
  • No background - no destaining necessary
  • Very simple application without heating, methanol, or acetic acid
  • Stabilised for a longer shelf life
Figure:SDS-PAGE with 3 µl Roti®-Mark Standard and samples.Staining duration: 20 mins.
Directions for use

The following protein amounts / band can be detected in the specified time (minutes):from 1 µg (3 min.)from 100 µg (15 min.)from 10 ng (60 min.)

The sensitivity of staining equals that of ROTI®Blue (Art. No. A152), so bands of 10 ng protein can be detected. ROTI®Blue quick is, therefore, approx. 5times more sensitive than standard Coomassie® staining solutions. ROTI®Blue quick staining is also fully compatible with usual down-stream applications like, membrane transfer & Western blotting, MS analysis (incl. MALDI) and protein gel elution.

Technical Information
Sensitivity 10 
Use Solution for rapid, highly specific staining of proteins. Destaining not required. Non-fixing staining, compatible with blotting and Proteomics. 

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