Description
Trypsin inhibitor, min. 10000 BAEE U/mg, Storage temp. +4 °C, CAS No. 9035-81-8, EC No. 232-906-9
Trypsin inhibitor inhibits Trypsin with high effiency, and, to a lesser extent, chymotrypsin. Additionally, there is a light inhibiting effect on plasmin, kallikrein, thrombin and a few other proteolytic enzymes.
Recommended final concentration: 1-100 µg/ml
Solutions of higher concentration may be hazy and slightly yellow-coloured.pH optimum: pH 7.6-8.0.
1 mg of lyophilisate inhibits at minimum 10.000 BAEE Units of trypsin.
Application in cell culture (stock solution 1 mg/ml): Following trypsination, add the same volume inhibitor solution as was used for trypsin. Zentrifuge cells (5 mins, 1000 rpm), and remove supernatant. Resuspend cells in medium and cultivate further.
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)