Description
ROTI®ProBind NC transfer membrane is a nitrocellulose blotting membrane with a pore size of 0,45 µm, supplied as ready-to-use sheets of 10 × 10 cm. It supports transfer of nucleic acids in Southern and Northern blotting procedures, providing a classic binding surface for immobilisation prior to hybridisation and detection. The 0,45 µm porosity is widely used for standard nucleic-acid transfer protocols and offers a practical balance of retention and flow. Each pack contains 25 sheets and weighs 0,354 kg, matching common mini-gel and cassette formats without additional cutting. This ROTI®ProBind NC membrane from manufacturer Carl ROTH is intended for molecular-biology and life-science laboratories performing routine blotting. Nitrocellulose remains a familiar matrix for many established transfer and detection workflows. Pre-cut 10 × 10 cm sheets simplify setup, reduce handling waste and help standardise transfer conditions across parallel experiments in teaching, research and service laboratories.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 25 sheets
- Membrane type: NC transfer membrane
- Material: Nitrocellulose
- Pore size: 0,45 µm
- Dimensions: 10 × 10 cm
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
DangerHazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®ProBind NC transfer membran, Nitrocellulose, 10 x 10 cm, Pore size 0.45 µm
What is this article?
ROTI®ProBind NC transfer membran, Nitrocellulose, 10 x 10 cm, Pore size 0.45 µm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiology lab supplies.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
What should I check before I order?
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.