Description
ROTI®ProBlot PVDF Blotting Sandwich is a polyvinylidene fluoride blotting sandwich prepared for transfer of nucleic acids to membranes in Southern and Northern blot applications. It features a pore size of 0,2 µm and a compact 70 x 84 mm format suited to smaller gel and transfer assemblies. Each unit supplies 20 pc(s), enabling standardised preparation of multiple blotting stacks in molecular biology laboratories. PVDF membranes are widely chosen when durable handling and stable nucleic-acid retention are needed during transfer and subsequent detection steps. Offered as a convenient pre-arranged sandwich from manufacturer Carl ROTH, the product supports efficient workflow setup without loose membrane cutting for the stated dimensions. Approximate pack weight is 0,090 kg. Integrate into established Southern and Northern protocols for transfer of nucleic acids, selecting this size and pore specification where the 70 x 84 mm footprint matches the gel system in use.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 20 pc(s)
- Membrane material: Polyvinylidene fluoride (PVDF)
- Pore size: 0,2 µm
- Dimensions: 70 x 84 mm
- Format: Blotting sandwich
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Questions about Blotting-Sandwich ROTI® ProBlot PVDF 0.2
What is this article?
Blotting-Sandwich ROTI® ProBlot PVDF 0.2 is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiology lab supplies.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
What should I check before I order?
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Luminix Health distributes this article from the heart of Europe. Delivery across Europe, two to five days when in stock. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.
