1 kbp DNA-Ladder, DNA-ladder (lyophil.) + gel loading, 50 µg, plastic

Catalog nr. Y014.1
Carl Roth

Volume: 50 µg
Value Packs: 1 piece
Price:
Sale price€50,45
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Description

1 kbp DNA-Ladder, DNA-ladder (lyophil.) + gel loading, buffer, not pre-dyed, Density (D) 1 g/cm³, Storage temp. -20 °C

Universal ladder for electrophoretic separation of fragments between 500 bp and 10 kb. Optimally suited for size determination of inserts, plasmids, and restricted DNA.The mass of the larger fragments was reduced, allowing to obtain clear and distinct bands with short running distances even for the large marker fragments.The terminal sticky EcoR I-restriction sites allow simple labelling of the marker fragments via end-labelling techniques.

Directions for use

The 1 kbp DNA-ladder is highly compatible with the 100 bp DNA-ladder (equalised: T833.1).

Fig.: 1.2 % agarose (NEEO, Art. No. 2267). Additional band at 125 bp.

Technical Information
Number of fragments 11 
Amount of traces ~200/100 µg 
Size range 500-10000 bp
Dispatch lyophilized 

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What is this article?

1 kbp DNA-Ladder, DNA-ladder (lyophil.) + gel loading, 50 µg, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Life Science & Microbiology.

Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.

Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.

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