Description
5-Bromo-4-chloro-3-indolyl phosphate, p-toluidine salt,min. 99 %, BioScience-Grade, Molar mass (M) 433,63 g/mol, Storage temp. -20 °C, CAS No. 6578-06-9, EC No. 229-506-1, Empirical formula C15H15N2O4BrClP
In combination with NBT ideal for colorimetric detection of alkaline phosphatase activity, in immunohistochemical procedures.
By enzymatic dephosphorylation BCIP is converted to indoxyl which subsequently tautomerizes to the ketone. In basic pH values, this ketone dimerizes and, in doing so, releases H+ ions. The dye precipitates in the direct vicinity of the phosphatase, hence staining this site in a weak blue colour. Enhencement and darkening of this colour is achieved by adding NBT (Art. No. 4421).
| Application | Western, in situ, Histochemistry |
| Enzyme | Alkaline phosphatase |
| Detection | Colour (brown) |
| Signal product | Precipitate (Blot) |
| Use | Western, in situ, histochimie |
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A microscopy stain binds a structure so it can be seen: haematoxylin on nuclei, eosin on cytoplasm, Gram reagents on a bacterial wall. A ready solution and a dry dye to be dissolved are different articles. The method names the stain. A substitute dye is a different method.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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