Description
Agarose NEEO ultra-quality ROTI®Garose, low electroendosmosis (EEO=0,05-0,13), CAS No. 9012-36-6, EC No. 232-731-8
Our agarose ROTI®Garose is a neutral polysaccharide which is obtained in many purification steps from the cell wall of certain algae, called Rhodophyceae. ROTI®Garose forms very clear gels with all standard running buffers and will result in a sharp and clear separation of your biomolecules. Extremely pure agarose with very low interference binding to staining reagents. ROTI®Garose produces a low background and high contrast appearance after staining.
- For clear and sharp bands
- Gels with high transparency
- Low background
- Suitable for all standard running buffers and high-speed buffer systems
- Compatible with all nucleic acid staining systems
- Non-toxic in cell immobilisation assays
- Of course DNase and RNase free
Chemically, agarose is a galactan which is capable of forming extremely solid gels, even at a low concentration. The pore size of the gels is determined by the concentration of the agarose used. Because there are no ionic groups in the gel, hydrophilic materials without any interaction with the gel matrix will also be separated according to their size. The high hysteresis of the agarose, that is the thermal stability after solidification, ensures that the gels remain reliably stable even during heatproducing running conditions.
- Excellent separative power for nucleic acids of 500 bp to 20 kb
- High gel strength for stable gels and good handling
- Extremely low EEO allows speedy and efficient separation of nucleic acid molecules
- Fast melting, non-foaming agarose
Figure: (left to right) Roth Lambda Hind III / phiX Hae III marker (Art. No. CP49.1) 1 µg, 1 kbp DNA-ladder (Art. No. Y014.1) 0,8 µg, 100 bp-DNA-ladder extended (Art. No. T835.1) 0,8 µg, pBR328 Mix I (Art. No. T146.1) 0,8 µg.
| Application | All standard applications, qualitative and quantitative gels, screening and blotting. Range 500 bp-20 kb |