anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plastic - Luminix Health
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anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plastic - Luminix Health

anti-DNA mouse monoclonal,, AC-30-10, 0.2 ml, plastic

SKU: 1NN5.1
Volume: 0.2 ml
Value Packs: 1 piece
Price:
€130,65

Description

anti-DNA mouse monoclonal,, AC-30-10, 100 µg/ml, affinity purified (made by PROGEN), Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +4 °C

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

  • High epitope affinity
  • Highly specific detection of the target protein
  • Unconjugated
  • Suitable for e.g. western blot, immunoprecipitation, ELISA & immunofluorescence

AC-30-10 stains DNA present in the nuclei and the mitochondria of cells and tissues. It is also a reliable positive control in assays for detection of DNA antibodies, especially for the detection of autoantibodies in autoimmune diseases. Due to the high sensitivity it can also be used for the detection of mycoplasma contamination in cell cultures. Binding characteristics: reacts with all forms of native and denatured DNA; reactive also with lambda DNA and synthetic DNA. Tested cultured cell lines: XLKE-A6 (Xenopus), RVF-SMC, PTK-2, HeLa.

Application

Dot Blot: Assay dependent (on nitrocellulose membrane, after baking at 70°C) Immunocytochemistry (ICC): 1:200 (0.5 μg/ml) Immunohistochemistry (IHC) - frozen: 1:10 (10 μg/ml) Immunohistochemistry (IHC) - paraffin: 1:10 (10 μg/ml, microwave treatment recommended)

Immunofluorescence analysis of HeLa cells with anti-DNA antibody. Fixation was performed using 3% paraformaldehyde for 10 min at RT. Cells were blocked with 5% BSA in PBST (PBS + 0.1% Tween 20) for 1 h at RT and permeabilized with 0.3% Triton-X 100 in PBS for 10 min at RT. The primary antibody anti-DNA mouse monoclonal, AC-30-10 (Cat. No. 690014) was diluted in blocking buffer (antibody concentration 0.5 μg/ml) and incubated over-night at 4°C. The secondary antibody goat anti-mouse IgM Alexa488 was also diluted in blocking buffer (antibody concentration 2.5 μg/ml) and incubated for 30 min at 37°C and 30 min at RT. Nuclear DNA was stained with DAPI in blue.
Not a medical device / Not an IVD product
Technical Information
Application ICC/IF IHC Dot Blot 
Host species mouse 
Clonality monoclonal 
Reactivity antibody species-independent 
Purification Size-exclusion chromatography  
Conjugate uncojugated 
Concentration 100 µg/ml 
Isotype IgM 
Clone AC-30-10 
Target double- and single-stranded DNA 

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