anti-Synaptopodin/SYNPO, mouse monoclonal, G1D4, 0.2 ml, plastic

This antibody portfolio includes a wide range of tissue- & tumor-specific primary antibodies for basic research, product development and analysis of a variety of biological questions. The monoclonal antibodies are perfectly applicable for all cell or tissue biology studies and for the analysis of predictive markers in research.

The antibody recognizes differentiated podocytes (glomerular visceral epithelial cells) in vivo and in vitro (weaker additional reaction with arterial endothelial cells), co-localization with alpha-actinin. Does not react with parietal cells. Reacts with a subset of exclusively telencephalic synapses. Differentiation-dependent expression during postnatal maturation of rat brain. Differentiation-dependent expression in cultured hippocampal neurons. The antibody reacts specifically with synaptopodin, a prolin-rich actin-binding protein with 2 binding sites for actin. Synaptopodin represents a new class of actin-binding proteins which has first been localized in podocytes and a subset of telencephalic postsynaptic densities. In human tissue synaptopodin has a molecular weight of 73.7 kDa and pI of 9.38 (calculated from sequence data); in mouse the corresponding data are 74 kDa, pI 9.27. In SDS-PAGE the antigen appears as 100 kDa polypeptide in brain and 110 kDa polypeptide in kidney (the difference might be attributed to posttranslational modifications). In Western blot analysis the antibody also reacts with a 44 kDa degradation fragment of synaptopodin.

Immunocytochemistry (ICC): assay dependentImmunohistochemistry (IHC) - frozen: 1:50-1:200 (0.25-1 μg/ml)Immunohistochemistry (IHC) - paraffin: 1:50-1:200 (0.25-1 μg/ml, microwave treatment recommended)Western Blot (WB): assay dependent