Description
ClickTech PCR Modification Kit 555, für die PCR, Storage temp. -20 °C
The PCR modification kits were developed for the production of fluorescently labelled DNA sequences.During strand extension in PCR, the ethynyl polymerase contained in the kit incorporates alkyne-modified nucleotides (EdUTP) into the new strand in addition to the native dNTPs. This results in modified PCR products, which can then be easily labelled with fluorescent dyes using the Click reaction. The labelled sequences can then be used for detection in subsequent hybridisation experiments.
The kit contains:
- dNTP Mix (10 mM)
- EdUTP (5 mM)
- baseclick Ethynyl Polymerase (2 U/µl)
- PCR Buffer (5x)
- 10x Activator2
- 5-TAMRA-PEG3-Azide
- Reactor 25
Technical Information
| λ | 555 nm |
| Markers | 5-TAMRA-PEG3-Azide |