Description
HPLC column NUCLEODUR®, 100-5 C8 Gravity, 250x4 mm
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This high density C8 phase based on high purity NUCLEODUR® silica gel with its endcapping suppresses any unwanted polar interactions between the silica surface and the sample, which makes “Gravity” particularly suitable for the separation of basic and other ionizable analytes. Due to the enhanced pH stability, LC/MS suitability and superior base deactivation (BDS) it is a perfect choice for method development and valuable for RP applications.
| Particle size | 5 µm |
| Pore size | 110 Å |
| L x Ø internal (column) | 250 x 4 mm |
| Column volume | 3.15 ml |
| Endcapped | yes |
| Eluent in column | Acetonitrile - Water |
| Phase | C8 Gravity |
| Surface chemistry | Base deactivated (BDS), High density monomeric octyl (C8, MOS) modification |
| USP listing | L7 |
| Separation principle | Reversed phase (RP) |
| Column shape | Analytical separation column |
| Column type | HPLC column |
| Particle type | Fully porous particles (FPP) |
| Specific surface according to BET | 340 m²/g |
| Pore volume | 0.9 ml/g |
| Temperature stability | 60 ° C |
| Interactions / retention mechanism | hydrophobic (van der Waals) |
| Pressure stability | 600 bar |
| pH stability | 1,0-11,0 |
| Recommended application(s) | In general compounds with ionizable functional groups such as basic pharmaceuticals and pesticides Analgesics Antidepressants Applications for extreme pH values (1-11) Anti-inflammatory drugs Herbicides Ideal for method development |
| Similar phases | NUCLEOSIL® C8 HD Xterra® RP8 / MS C8 Luna® C8 Zorbax® Eclipse XDB-C8 |
| Application Database (Macherey-Nagel) | HPLC |
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Questions about HPLC column NUCLEODUR®, 100-5 C8 Gravity, 250x4 mm, 1 unit(s)
What is this article?
HPLC column NUCLEODUR®, 100-5 C8 Gravity, 250x4 mm, 1 unit(s) is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in HPLC columns, inside HPLC columns & accessories, in Chromatography equipment.
An HPLC column is specified by the stationary phase, the particle size, the length and the internal diameter. A C18, 5 µm, 150 × 4.6 mm column is a different article from a C18, 3 µm, 100 × 2.1 mm column. The smaller bore belongs on a lower flow. A column has a direction and a pH range.
An HPLC column is a tube packed, or coated, with a stationary phase. The sample is carried through it by a liquid, and the compounds leave at different times. The phase, the particle size, the length and the internal diameter decide the separation. A guard column is a short sacrificial piece in front of the analytical column. Fittings and tubing have a bore that either preserves the peak or smears it. Compound names follow IUPAC. Data are on PubChem. Method grades often cite the European Pharmacopoeia or USP.
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The category is HPLC columns & accessories. The sub-category is HPLC columns. The catalogue is Chromatography equipment.
Shelves beside this one are HPLC fittings, tubing & inline filters, HPLC guard columns and HPLC accessories.
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