Description
MES monohydrate Pufferan® is a high-purity buffer salt with a minimum assay of 99 %, intended for biological and biochemical buffer preparation. Identified by CAS number 145224-94-8 and empirical formula C₆H₁₃NO₄S · H₂O, it has a molar mass of 213,2 g/mol and a boiling point of 342,9 °C; the EG number is 224-632-3. This monohydrate form of MES supports reliable pH control in the working range typical of MES-based systems Researchers employ it when preparing stock and working buffers for protein handling, cell-related workflows and other applications that require stable, well-defined buffering capacity. Offered from manufacturer Carl ROTH within the Pufferan® line, the material combines clearly stated composition data with practical pack sizing for routine and research settings. Its documented molar mass, purity level and physical constants help laboratories standardise buffer recipes and maintain reproducible conditions across experiments.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Appearance: white powder
- Assay (titr.): ≥99,0 %
- pH value (1 % in H2O): 3,5-4,1
- Loss on drying: 7,9-8,9 %
- Solubility (0,1 M in H2O): clear, colorless solution
- 260 nm: ≤0,04
- 280 nm: ≤0,02
- CAS number: 145224-94-8
- Pack sizes: 50 g, 100 g, 500 g, 1 kg
- EG nr.: 224-632-3
- Purity: min. 99 %
- Empirical formula: C₆H₁₃NO₄S · H₂O
- Molar mass: 213,2 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about MES monohydrate, Pufferan®, min. 99 %, 50 g, plastic
What is this article?
MES monohydrate, Pufferan®, min. 99 %, 50 g, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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