Description
p-Nitrotetrazolium blue chloride, min. 98 %, p.a., Molar mass (M) 817,65 g/mol, Storage temp. +4 °C, CAS No. 298-83-9, EC No. 206-067-4, Empirical formula C40H30Cl2N10O6
Used in combination with BCIP-toluidine salt (X-phosphate) for detection of alkaline phosphatase activity by producing an indigo dye precipitate. Colour enhancer for staining with alkalic phosphatase.
During dimerisation of ketonized X-Phos, H+ ions are set free which reduce NBT to purple-coloured diformazan. Both dyes precipitate in the direct vicinity of the phosphatase, hence staining this site in a dark violet. Store at +4 °C. Please also see assay protocol for use of BCIP/NBT in immunoblot procedures.
| Application | Western, in situ, Histochemistry |
| Enzyme | Alkaline phosphatase |
| Detection | Colour (brown-violet) |
| Signal product | Precipitate (Blot) |
Recently viewed
You may also like
Questions about p-Nitrotetrazolium blue chloride, min. 98 %, p.a., 100 mg, glass
What is this article?
p-Nitrotetrazolium blue chloride, min. 98 %, p.a., 100 mg, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein biology (other), inside Protein biology & Western blot, in Life Science & Microbiology.
This shelf is the protein-biology article that is not a membrane and not an extraction kit: stains for gels, buffers, small tools of a protein bench. The method, a gel stain or a quantitation assay, still chooses the bottle.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Protein biology (other). The catalogue is Life Science & Microbiology.
Shelves beside this one are Blotting membranes & papers and Protein extraction, purification & markers.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.