Description
PIPES is a high-purity zwitterionic buffer reagent supplied as PUFFERAN® grade with a minimum assay of 99 %, widely used in protein biology and related life-science applications. It has the empirical formula C₈H₁₈N₂O₆S₂, a molar mass of 302,37 g/mol, CAS number 5625-37-6 and EG number 227-057-6. is sized for preparing stock and working buffers in protein isolation, purification and characterisation workflows. This buffer salt from manufacturer Carl ROTH provides reliable pH control in the near-physiological range and is a standard choice where Good’s buffers are preferred. High minimum purity supports reproducible buffer composition for sensitive biomolecular work. Laboratories can dissolve and titrate the free acid form to the required working pH for chromatography, extraction or assay buffers. Documented CAS, EG and molar-mass data simplify specification and traceability in protein purification and reagents-for-isolation protocols.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Appearance: white powder
- Assay (titr.): ≥99 %
- Water: ≤1.0 %
- Solubility: clear, soluble
- 280 nm: ≤0.1
- 260 nm: ≤0.1
- CAS number: 5625-37-6
- Pack sizes: 25 g, 100 g, 250 g, 500 g
- EG nr.: 227-057-6
- Purity: min. 99 %
- Molar mass: 302,37 g/mol
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about PIPES, PUFFERAN®, min. 99 %, 25 g, plastic
What is this article?
PIPES, PUFFERAN®, min. 99 %, 25 g, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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