Protino 96 Ni-NTA (1x96)

Catalog nr. 7454251
Macherey-Nagel

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Sale price€226,36
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Description

Protino 96 Ni-NTA (1×96) is a ready-to-use 96-well kit for affinity purification of His-tag proteins. Each pack provides material for 1×96 preparations and includes a Protino Purification Plate, Protino Ni-NTA Agarose and the corresponding buffers, enabling parallel processing of multiple samples in a compact plate format. Nickel-NTA chemistry supports selective binding of polyhistidine-tagged recombinant proteins from clarified lysates under standard IMAC workflows used in molecular biology and protein biochemistry laboratories. The plate-based layout is suited to centrifugation- or vacuum-assisted handling and helps streamline wash and elution steps when screening expression clones or preparing small-scale protein batches. Supplied from manufacturer Macherey-Nagel, this configuration is intended for laboratories that need a single 96-prep set without excess inventory. Components are matched for coordinated use within the Protino Ni-NTA system, supporting consistent binding and recovery across wells. The kit is not classified as dangerous goods under transport regulations and is offered as a complete package for His-tag protein purification workflows.

This article is part of Protein extraction, purification & markers, in Protein biology & Western blot.

Key features

  • Pack size: 1 pkg
  • Storage temperature: 6 - 7 °C
  • Format: 1 × 96 preps
  • EAN: 4046681153136
  • Technical Information Sheet: PDF
  • Safety Data Sheet: PDF

Compliance & Safety Information

GHS02 Warning

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Questions about Protino 96 Ni-NTA (1x96)

What is this article?

Protino 96 Ni-NTA (1x96) is the article on this page. Luminix Health distributes Macherey-Nagel for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Life Science & Microbiology.

Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.

Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.

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