Description
ROTI®Cell DMEM , F12, sterile, w/o glutamine, with pyruvate and, HEPES, ready-to-use, CELLPURE®, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +2 to +8 °C
Sterile
DMEM : F12 is a widely used basal medium for a variety of mammalian cells. It is a 1:1 mixture of Ham’s F12 and DMEM. In this way, the richness of Ham’s F12 in vitamins and trace elements has been combined with the high nutrient concentration of DMEM.DMEM : F12 is now the basis of many serum-free media.
When working without FBS, growth factors, hormones and lipids, which are essential for cell growth, must also be added to the medium.
This modification of DMEM : F12 contains pyruvate and 15 mM HEPES, but no L-glutamine.
Technical Information
| Use | Cell culture |
| Instructions for use, addition | Store at +2 °C to +8 °C protected from light. Once opened, use within 6-8 weeks. |
| Additional info | The detailed composition can be found in the specification. |