Description
ROTI®Cell DMEM : F12 is a classical cell culture medium for life-science and cell biology work, supplied sterile and ready-to-use. The formulation is prepared without glutamine and contains pyruvate and HEPES, matching protocols that require a DMEM/F12 basal medium with this additive profile. As a CELLPURE® grade product it is filled in a 500 ml plastic pack convenient for bench use and cold-room stock rotation. Density is approximately 1 g/cm³ and the boiling point is approximately 100 °C, in line with aqueous culture media. This medium from manufacturer Carl ROTH belongs to the classical media range used for routine cultivation, expansion, and maintenance tasks. Ready-to-use sterile presentation reduces preparation steps before medium exchange or culture initiation. The 500 ml unit quantity offers a practical balance between handling ease and working volume for research groups. Laboratories standardising on DMEM/F12 blends can integrate this ROTI®Cell article where a glutamine-free, pyruvate- and HEPES-containing sterile medium is specified in cell culture workflows.
This article belongs on Cell culture media & sera, in Cell culture.
Key features
- Glucose: 3.151 g/l
- HEPES: 15 mM
- pH value: 7.0-7.6
- Osmolality: 285-315 mOsm/kg
- Endotoxins: ≤1.0 EU/ml
- Sterility: complies
- Pack size: 500 ml plastic
- Density: ~1 g/cm³
- Storage temperature: +2 to +8 °C
- Grade: CELLPURE®
- Formulation: w/o glutamine, with pyruvate and HEPES
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about ROTI®Cell DMEM , F12, sterile, w/o glutamine, with pyruvate and, 500 ml, plastic
What is this article?
ROTI®Cell DMEM , F12, sterile, w/o glutamine, with pyruvate and, 500 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Cell culture media & sera, inside Cell culture, in Life Science & Microbiology.
Cell-culture medium is the liquid a cell line grows in. Serum, often fetal bovine, supplies what the basal medium does not. With or without glutamine, with or without phenol red, and the serum origin are different products. Serum is a cold-chain article and is never part of free standard delivery.
Cell culture grows cells outside the body. The medium, often with serum, supplies what the flask does not. A flask or a dish is the vessel, treated so the cells attach or left untreated so they float. Contamination by microbes, or by another cell line, is the ordinary failure. Cold-chain articles in this work, including serum, are never part of free standard delivery. Lines are catalogued by ATCC. Papers are on PubMed. Practice is summarised by the WHO laboratory biosafety topic.
Which category should I open next?
The category is Cell culture. The sub-category is Cell culture media & sera. The catalogue is Life Science & Microbiology.
Shelves beside this one are Cell culture accessories, Cell culture flasks, Cell culture plates & dishes and Cell strainers, inserts & scrapers.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.

