Description
ROTI®Free Stripping Buffer 2.0, ready-to-use, Density (D) ~1 g/cm³, Boiling point (bp) ~100 °C, Storage temp. +15 to +25 °C, UN-Nr. 1760
Stripping buffer for efficient removal of primary and secondary antibodies, as well as chemoluminescence staining, from Western blot membranes. Due to the low pH of the buffer, the antibody conformation is altered, therefore gently removing all molecules bound to the epitopes (Legocki et. al. (1981) Analyt. Biochem. 111:385-92). Robust formulation without β-mercaptoethanol or DTT for use at room temperature. Suitable for nitrocellulose and PVDF membranes.
- Effective
- Gentle
- Odour-free
- Simple application w/o heating
- Cost-efficient
Following rinsing steps in PBST- or TBST buffer, the membrane is incubated in ROTI®Free Stripping-Puffer 2.0 for 15-60 mins. at room temperature. After washing it again, the membrane may be blocked and probed anew. The particular time needed for complete stripping depends on the respective antibody/epitope pairing.
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)