Description
ROTI®Free Stripping Buffer 2.2 plus is a ready-to-use reagent formulated for antibody stripping in Western blot and related immunoassay workflows. This buffer from manufacturer Carl ROTH supports efficient removal of primary and secondary antibodies from blotted membranes so that membranes can be reprobed without preparing fresh stripping mixtures at the bench. It is suited to Western blot, blotting applications linked to ELISA formats, and multi-target detection strategies where sequential probing is required. The ready-to-use presentation reduces preparation time and handling steps during protein analysis. Physical properties aid laboratory risk assessment under UN number 1760. Research groups performing signal optimisation, membrane reuse, and multi-probe protocols benefit from a consistent stripping reagent that integrates into standard blotting pipelines. size is practical for routine laboratory throughput in molecular biology and protein biochemistry environments.
This article is part of Protein extraction, purification & markers, in Protein biology & Western blot.
Key features
- Pack sizes: 250 ml, 1 l
- Density: 1,01 g/cm³
- Boiling point: ~100 °C
- Format: Ready-to-use
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
Warning
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Protein biology & Western blot
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Protein extraction, purification & markers
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Questions about ROTI®Free StrippingPuffer 2.2 plus, ready-to-use, 250 ml, glass
What is this article?
ROTI®Free StrippingPuffer 2.2 plus, ready-to-use, 250 ml, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Protein extraction, purification & markers, inside Protein biology & Western blot, in Life Science & Microbiology.
Extraction reagents break the cell and keep the protein soluble. Purification resins and columns, often an affinity tag such as His, pull one protein out. A marker is a set of known masses run in the next lane. The mass range of the marker has to cover the protein being sought.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Protein extraction, purification & markers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Blotting membranes & papers and Protein biology (other).
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.