Description
ROTI®Load DNA 1x (with glycerol), 1x DNA gel loading buffer,ready-to-use, DNase-free, Density (D) 1 g/cm³, Boiling point (bp) 100 °C, Storage temp. -20 °C
The Carl ROTH gel loading buffers are special buffer mixtures for nucleic acid electrophoresis, which are used as sample additives during gel loading. The gel loading buffers are ready-to-use solutions, which may be used directly.
ROTI®Load DNA buffer solutions contain Tris, sodium acetate and EDTA, with additional tracking dyes. For increasing the density when loading the gel, they are available in three different variations - with glycerol, ficoll and saccharose.
ROTI®Load DNA can either be added directly to the dissolved sample or the precipitated RNA can be solubilised directly in ROTI®Load DNA. ROTI®Load DNA gel loading buffer is usually used as 1x concentrated solution.
In order to cover the main size reange needed in gel electrophoresis, ROTI®Load DNA 1x (with glycerol) contains bromophenol blue and xylene cyanol (see lane 1 in the product image).
Glycerin is the standard reagent for increasing sample density.
Each batch is tested for its functionality in electrophoresis and proved to be DNAse-free.
| Dye | Bromphenol blue, Xylene cyanole |
| Incl. DNA staining | no |
| Use | Standard applications, pelleted DNA |