Description
ROTI®Nylon 0.45 is a neutrally charged nylon transfer membrane supplied as ready-to-use sheets measuring 20 × 20 cm, with a pore size of 0,45 µm. It is designed for transfer of nucleic acids to membranes in Southern and Northern blotting and related molecular biology applications. The neutral charge supports controlled binding of DNA and RNA during immobilisation, hybridisation and detection, while the 0,45 µm pores aid fragment retention and buffer exchange in capillary, vacuum or electrotransfer systems. Each pack contains five sheets, giving a convenient format for standard gel sizes without extensive cutting. Laboratories can load gels, assemble transfer stacks and process blots with consistent membrane dimensions from run to run. This transfer membrane from manufacturer Carl ROTH is suited to routine nucleic acid blotting, probe hybridisation workflows and method optimisation where a reliable nylon support is required. Pre-cut 20 × 20 cm sheets streamline setup, help standardise transfer area and support reproducible Southern and Northern blot results across multiple experiments.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 5 sheets
- Product type: Nylon transfer membrane
- Charge: Neutral charged
- Pore size: 0,45 µm
- Format: Sheets
- Dimensions: 20 × 20 cm
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Questions about ROTI®Nylon 0.45, transfer membrane, neutral charged, sheets, 20 x 20 cm
What is this article?
ROTI®Nylon 0.45, transfer membrane, neutral charged, sheets, 20 x 20 cm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiology lab supplies.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.