Description
ROTI®Pol TaqS Mix (2x), ready-to-use, 2x conc., Density (D) ~1 g/cm³, Storage temp. -20 °C
For PCR. Recombinant, heat stable DNA polymerases from the thermophilic bacteria Thermus aquaticus.
ROTI®Pol, the series of DNA polymerases is the optimal choice for all PCR cycling protocols, as being performed in, for instance, analysis of cloning efficiency, for gene fishing, in routine screening processes, educational assays and much more. In combination with our specially designed buffers, the ROTI®Pol DNA polymerases deliver specific and reproducible PCR amplification with a wide range of PCR templates.
Optimized pre-mixed 2x PCR solution containing the recombinant TaqS DNA polymerase from the thermophilic bacterium Thermus aquaticus, dNTPs, MgCl2, and all other components required for PCR except primers and template DNA. ROTI®Pol TaqS Mix (2x) is recommended for use in all standard PCR applications.PCR assaying with ROTI®Pol TaqS Mix (2x) master mix not only reduces contamination risks, but is also time-saving, highly reproducible and very easy to prepare. ROTI®Pol TaqS Mix (2x) can be applied to all standard Taq-based cycling protocols and is, therefore, the optimal choice for high throughput PCR as being performed in, for instance, analysis of cloning efficiency or colony screening, for routine screening processes, and for student's courses.Due to the optimized composition of the master mix, the TaqS polymerase delivers specific PCR amplification of good yield with a wide range of PCR templates. ROTI®Pol TaqS Mix (2x) is able to amplify PCR products up to 3 kb with genomic DNA, and is appropriate for use with pure DNA solutions, cDNA, and bacterial colonies as templates. The use of the colourless PCR Master mix is particularly recommended when direct fluorescence or absorbance readings are required. The TaqS polymerase included in the master mix possesses a 5’ → 3’ polymerase- as well as a 5’-flap endonuclease activity, and generates a 3’dA (adenine)-overhang which may well be used for TA-cloning purposes.
2 or 10 vials, respectively, with 1 ml each of ROTI®Pol TaqS Mix (2x) containing TaqS polymerase, 0,4 mM each dNTP, and 4 mM MgCl2 in 2x reaction buffer
| Application | Standard PCR |
| Amplicon ends | 3'dA |
| Master mix | colourless |
| Polymerase | Standard Taq polymerase |
| Use | Standard PCR |
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Questions about ROTI®Pol TaqS Mix (2x), ready-to-use, 2x conc., 2 ml, plastic
What is this article?
ROTI®Pol TaqS Mix (2x), ready-to-use, 2x conc., 2 ml, plastic is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in PCR, qPCR & nucleic acid analysis reagents, inside Molecular biology, in Life Science & Microbiology.
PCR and qPCR reagents are the polymerase, the nucleotides, the buffer and often a ready master mix, plus the dyes a real-time instrument reads. Hot-start enzymes and proofreading enzymes are different tools. A master mix already contains nucleotides. Adding them again is a failed reaction.
Molecular biology here is the handling of DNA and RNA. Extraction kits break the cell and then separate the nucleic acid from protein and from the other nucleic acid. PCR and qPCR reagents copy a chosen sequence so it can be seen. The enzymes and the nucleotides are what the copy depends on. Nuclease contamination destroys the sample before the kit can save it. Sequences are on NCBI Nucleotide. Papers are on PubMed.
Which category should I open next?
The category is Molecular biology. The sub-category is PCR, qPCR & nucleic acid analysis reagents. The catalogue is Life Science & Microbiology.
Shelves beside this one are DNA & RNA extraction kits and Molecular biology reagents (other).
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