ROTI®Prep RNA MINI, 250 preparations, for molecular biology, cardboard

Roti^®Prep RNA MINI has been developed for the isolation of total RNA from various sources.The isolation is carried out through the proven and reliable spin column technique, which is very easy to handle and needs only few steps.

One of the critical points in RNA extraction is the presence of endogenous RNases, which can lead to high losses very early in extraction of the (in comparison to DNA) very sensitive RNA. Therefores the lysis buffer has been specially optimised to inactivate RNases, with highest efficiency, already at the early stage of the extraction.Furthermore, in order to get a high-pure total RNA, one particularly for this purpose developed prefilter is used. This filter exclusively serves for binding and targeted removal of DNA impurities, and is discarded after use with the bound DNA. Thus, it is possible to omit application of DNase I.Total RNA is then obtained from the DNA-free eluate through a simple “Binding-Washing-Elution” process.In each preparation, up to 100 μg highly pure total RNA may be isolated. One preparation takes (excluding lysis) approx. 10 mins.Isolated RNA is free of DNA and enzyme inhibitors, and may directly be applied to RT-PCR or other downstream applications.

Lysis Buffer LSR, Washing Buffer WSA, Washing Solution WSL, RNase-free Water, spin filters (blue), spin columns (violet), collection tubes, elution tubes, detailed instructions-for-use.Contents of this Kit may not be bought separately.Please order ethanol 99,8 % (p.a., Art. No. 9065) and 70 % (p.a., Art. No. T868) separately.