Description
ROTI®Prep Viral RNA/DNA MINI, for molecular biology, 10 preparations, Density (D) ~1 g/cm³, Storage temp. +15 to +25 °C, UN-Nr. 3316
ROTI®Prep Viral RNA/DNA MINI has been developed for the isolation of viral DNA and RNA from various kinds of source material.The isolation is carried out via the proven and reliable spin column technique, which is very easy to handle and needs only few steps.
- Preparation by well-known mini spin-column system
- Fast, easy and efficient
- Preparation within approx. 25 minutes
- Elution in 60 μl
The kit enables the simultaneous extraction of viral ssDNA, dsDNA and ssRNA. The purification happens by using a carrier RNA, which engenders a higher binding of the nucleic acid (resp. its complex with the carrier RNA) on the spin filter and thus a higher recovery rate.Furthermore, in order to achieve a maximum of nucleic acid purity for each source material, 5 specifically optimised protocols are provided.Thus, within approx. 25 mins high-pure viral nucleic acid is eluted, free from inhibitors. This nucleic acid may directly be applied to further downstream applications, like RT-PCR, PCR, packaging etc.
- Tissues and biopsies (up to 20 mg)
- Cell culture supernatants, serum, plasma, cell-free body fluids (up to 400 μl)
- Swabs
Lysis Buffer LSV, Binding Buffer BSN, Carrier CS, RNase-free Water, Proteinase K, Washing Buffer WSA, Washing Solution WSL, spin columns, collection tubes, elution tubes, detailed instructions-for-use.Contents of this Kit may not be bought separately.Please order ethanol 99,8 % (p.a., Art.-No. 9065) separately.
| Basic material | Tissue, biopsies, plasma, serum, cell-free biological fluids smears |
| Quantity of material | Tissue and biopsin <20 mg Supernatant from cell cultures, serum, plasma, cell-free body fluids <400 μl |
| Preparation time | approx. 25 min |
| Version | Mini Prep |
| Automatic use | no |
| Elution volume | 60 µl |
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about Roti®-Prep Viral RNA/DNA MINI - 250 pcs, for molecular biology, 1 kit, cardboard
What is this article?
Roti®-Prep Viral RNA/DNA MINI - 250 pcs, for molecular biology, 1 kit, cardboard is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in DNA & RNA extraction kits, inside Molecular biology, in Life Science & Microbiology.
An extraction kit lyses the sample and then binds the nucleic acid, usually on a column or on a bead, and washes the rest away. Kits for blood, tissue, soil or a PCR cleanup are different chemistries. Yield and the size of fragments they keep are in the protocol. An RNA kit has to protect against RNase.
Molecular biology here is the handling of DNA and RNA. Extraction kits break the cell and then separate the nucleic acid from protein and from the other nucleic acid. PCR and qPCR reagents copy a chosen sequence so it can be seen. The enzymes and the nucleotides are what the copy depends on. Nuclease contamination destroys the sample before the kit can save it. Sequences are on NCBI Nucleotide. Papers are on PubMed.
Which category should I open next?
The category is Molecular biology. The sub-category is DNA & RNA extraction kits. The catalogue is Life Science & Microbiology.
Shelves beside this one are Molecular biology reagents (other) and PCR, qPCR & nucleic acid analysis reagents.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
How does ordering and shipping work?
Luminix Health distributes this article from the heart of Europe. Articles in stock ship within 2–5 business days. Standard delivery is free from €250 excl. VAT. Cold-chain goods and dangerous goods under ADR are never included in that free delivery. The terms are in the Shipping Policy. The full brand index is on shop by brand. Who we are is on about us.
Which other categories should I open?
The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.