Description
ROTI®ProBind PVDF transfer membrane is a polyvinylidene fluoride blotting membrane with a pore size of 0,2 µm, supplied as a continuous roll measuring 30 × 300 cm. It is designed for transfer of nucleic acids to solid supports in Southern and Northern blotting workflows, where uniform pore structure and reliable binding support hybridisation and detection steps. The fine 0,2 µm porosity suits applications that require strong retention of smaller nucleic acid fragments during capillary or electrophoretic transfer. Pack size is 1 roll with a weight of 0,680 kg, so laboratories can cut custom sheets to match diverse gel formats and transfer assemblies. This ROTI®ProBind membrane from manufacturer Carl ROTH is established for routine molecular-biology blotting and nucleic-acid immobilisation. The PVDF matrix offers mechanical strength and chemical resistance through wetting, transfer, washing and probing. Roll format is practical for multi-format or higher-throughput setups that need flexible membrane dimensions without pre-cut sheet constraints.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 1 roll
- Membrane type: PVDF transfer membrane
- Material: Polyvinylidene fluoride (PVDF)
- Pore size: 0,2 µm
- Dimensions: 30 × 300 cm
- Weight: 0,680 kg
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Questions about Transfer membrane ROTI® ProBind PVDF 0.2, 300 × 30 cm
What is this article?
Transfer membrane ROTI® ProBind PVDF 0.2, 300 × 30 cm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Life Science & Microbiology.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Life Science & Microbiology.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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