Description
ROTI®ProBlot NC Blotting Sandwich is a nitrocellulose blotting sandwich for laboratory transfer of nucleic acids in Southern and Northern blot applications. Each sandwich measures 70 × 84 mm and features a pore size of 0,45 µm, aligning with compact gel formats and protocols that specify standard NC porosity. The unit pack holds 20 pc(s) and weighs 0,704 kg, providing a practical stock for repeated blotting runs. This ROTI®ProBlot sandwich from manufacturer Carl ROTH is aimed at molecular biology workflows where nitrocellulose membranes are used for nucleic acid immobilisation after electrophoretic separation. Pre-assembled, pre-cut pieces simplify cassette loading and help keep blot geometry consistent between experiments. The 0,45 µm pore size suits many routine nucleic-acid transfer methods that balance retention with buffer flow through the membrane. Use in Southern and Northern blotting and related membrane transfer procedures requiring 70 × 84 mm nitrocellulose sandwiches at 0,45 µm.
This article is part of Blotting membranes & papers, in Protein biology & Western blot.
Key features
- Pack size: 20 pc(s)
- Product type: NC blotting sandwich
- Material: Nitrocellulose
- Pore size: 0,45 µm
- Dimensions: 70 × 84 mm
- Technical Information Sheet:
- Safety Data Sheet:
Compliance & Safety Information
Danger
Hazard Statements (H-Phrases)
Precautionary Statements (P-Phrases)
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Questions about ROTI®ProBlot NC Blotting Sandwich, Nitrocellulose, 70 x 84 mm, Pore size 0.45 µm
What is this article?
ROTI®ProBlot NC Blotting Sandwich, Nitrocellulose, 70 x 84 mm, Pore size 0.45 µm is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Blotting membranes & papers, inside Protein biology & Western blot, in Microbiology lab supplies.
A blotting membrane, nitrocellulose or PVDF, binds proteins transferred out of a gel. Pore size, 0.2 µm or 0.45 µm, and the format, roll or pre-cut, are the choices. PVDF is activated in methanol. The papers and the sponges around it in the cassette are part of the same stack.
Protein work separates a mixture, then asks which protein is which. Extraction and purification take the protein out of the cell. A marker shows the masses. A Western blot moves the separated proteins onto a membrane and probes them with an antibody. The membrane, nitrocellulose or PVDF, has to bind the protein and survive the washes. Antibodies and methods are in PubMed. Reference material is at ATCC.
Which category should I open next?
The category is Protein biology & Western blot. The sub-category is Blotting membranes & papers. The catalogue is Microbiology lab supplies.
Shelves beside this one are Protein biology (other) and Protein extraction, purification & markers.
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