Description
Safranin O solution is a microscopy stain based on the cationic dye Safranin O (CAS number 477-73-6, EG nr. 207-518-8), widely used as a counterstain in Gram procedures and for general histological contrast. The preparation is supplied in a 500 ml glass bottle and exhibits a density of 1 g/cm³ with a boiling point of 100 °C. In Gram staining sequences it colours Gram-negative organisms red to pink after decolourisation, while also serving in botanical and cytological work to highlight nuclei and cell walls. This solution from manufacturer Carl ROTH is intended for routine laboratory microscopy where a ready aqueous Safranin O reagent is required. The defined physical properties support consistent staining intensity and handling under standard laboratory conditions. Suitable for clinical microbiology, teaching laboratories and research histology benches that need a reliable counterstain in a convenient glass pack size for repeated use.
This article belongs on Microscopy stains & staining solutions, in Dyes, stains & indicators.
Key features
- CAS number: 477-73-6
- Pack size: 500 ml glass
- EG nr.: 207-518-8
- Density: 1 g/cm³
- Boiling point: 100 °C
- Technical Information Sheet:
- Safety Data Sheet:
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Questions about Safranin O solution, for microscopy, 500 ml, glass
What is this article?
Safranin O solution, for microscopy, 500 ml, glass is the article on this page. Luminix Health distributes Carl Roth for laboratory use. It sits in Microscopy stains & staining solutions, inside Dyes, stains & indicators, in Lab chemicals.
A microscopy stain binds a structure so it can be seen: haematoxylin on nuclei, eosin on cytoplasm, Gram reagents on a bacterial wall. A ready solution and a dry dye to be dissolved are different articles. The method names the stain. A substitute dye is a different method.
A dye or a stain binds, or changes colour, so that something invisible becomes visible. An indicator changes at a pH or a redox point. A fluorescent dye absorbs one wavelength and emits another, which is how a microscope or a plate reader picks it out. The excitation and emission, or the transition interval, are the specification. The colour name on the label is not. Structures are on PubChem under IUPAC names. Methods are in the PubMed literature.
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The category is Dyes, stains & indicators. The sub-category is Microscopy stains & staining solutions. The catalogue is Lab chemicals.
Shelves beside this one are Fluorescent dyes & labels and Dyes & pigments.
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