Description
TAE Electrophoresis Buffer X50, 500 ml is a concentrated Tris-Acetate-EDTA solution formulated for agarose gel electrophoresis (AGE) of nucleic acids such as DNA and RNA. The 50X concentrate serves both as a running buffer for preparative separations and as a component in the preparation of agarose and polyacrylamide gels. Before use, the concentrate must be diluted to a working concentration of 1X; fresh 1X buffer is recommended for each electrophoresis run to maintain performance. Supplied in a 500 ml pack with a net weight of 0,500 kg, this buffer from manufacturer Sartorius supports reliable nucleic-acid separation in molecular biology laboratories. Users should gently swirl the bottle to ensure homogeneity, verify that the cap is secure, and apply aseptic technique under a laminar-flow hood when following established protocols. TAE remains a standard choice for analytical and preparative gel electrophoresis workflows that require consistent ionic strength and buffering capacity for DNA and RNA fragments.
This article is part of Biological buffers (Tris, HEPES, MOPS), in Buffers & pH solutions.
Key features
- Pack size: 1 pc
- Storage temperature: Room Temperature
- Buffer type: Tris-Acetate-EDTA (TAE)
- Concentration: 50X concentrate
- Volume: 500 ml
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Questions about TAE Buffer 50X 500ML
What is this article?
TAE Buffer 50X 500ML is the article on this page. Luminix Health distributes Sartorius for laboratory use. It sits in Biological buffers (Tris, HEPES, MOPS), inside Buffers & pH solutions, in Lab chemicals.
Tris, HEPES, MOPS and their relatives hold pH in a range that cells and proteins tolerate, with a pKa near the working pH. The useful range is roughly one unit either side of the pKa. A phosphate buffer and Tris are not interchangeable in a method that names one of them, because the ion itself has effects.
A buffer resists a change in pH when a little acid or base is added. It does that because a weak acid and its conjugate base are both present. A pH buffer used to calibrate a meter has a stated value at a stated temperature. A biological buffer such as Tris or HEPES is chosen because it holds the pH of a living system and does not poison it. Conductivity standards are the sister articles for a conductivity meter. The ions are on PubChem. Electrode calibration practice sits with EURAMET and BELAC.
Which category should I open next?
The category is Buffers & pH solutions. The sub-category is Biological buffers (Tris, HEPES, MOPS). The catalogue is Lab chemicals.
Shelves beside this one are pH buffers & conductivity standards and Buffers (other).
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