Description
Amersham(TM) Protran® 0.45 µm NC, roll, 300 mm x 4m, Flash point (flp) 13 °C
Transfer membrane for most nucleic acid and protein immobilising techniques
Nitrocellulose should only conditionally be used for electrophoretic transfer of nucleic acids, because of the high salt concentrations required for the transfer. Membranes of 0,45 μm pore size allow for DNA transfer (minimum 300 bp) and protein transfer (minimum 20 kDa).
Southern, Northern, Western transfer, colony and plaque hybridisation, immunoblots, nucleic acid and protein dot-blot, slot-blot, electrophoretic transfer of proteins.Detection systems are radioactivity, chemoluminescence, colour
In practical roll format for maximum flexibility in cutting.
| Dimensions | 400 × 30 cm |
| Version | roll |
| Pore size | 0,45 µm |
| Type | Protran® NC 0,45 |
| Bond | electrostatic, hydrophobic |
| Immobilisation | UV, baking (max. 80 °C) |
| Binding capacity | 86-110 µg/cm² |
Compliance & Safety Information
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Questions about Amersham(TM) Protran® 0.45 µm NC, roll, 300 mm x 4m, 1 roll(s)
What is this article?
Amersham(TM) Protran® 0.45 µm NC, roll, 300 mm x 4m, 1 roll(s) is the article on this page. Luminix Health distributes Cytiva for laboratory use. It sits in Membrane filters, inside Filtration, in Consommables.
A membrane filter is the disc itself, without a syringe housing. Pore size, polymer and diameter are the specification, and the disc has to sit in a holder. Gridded membranes are for counting colonies. A membrane and a filter paper of the same diameter are not interchangeable.
Filtration holds back what is larger than the pores and lets the rest through. Paper is the ordinary laboratory filter. A membrane has a stated pore size and a polymer that must survive the solvent. A syringe filter is that membrane in a housing on a syringe. Glass fibre takes a higher dirt load. Ultrafiltration holds back macromolecules and passes salts. The pore size, the chemical compatibility and the volume are the three choices. Laboratory apparatus around filtration is indexed under ISO laboratory apparatus.
Which category should I open next?
The category is Filtration. The sub-category is Membrane filters. The catalogue is Consommables.
Shelves beside this one are Filter paper, Sintered glass filter plates & discs, Syringe filters, Filter cartridges & capsules, Glass & quartz fibre filters, Bottle-top & vacuum filtration units, Ultrafiltration & concentrators, Filtration accessories, Extraction thimbles and Dialysis tubing & devices.
What should I check before I order?
The technical information on this page is the manufacturer's sheet. Read that, not a rewritten specification.
When this page shows a safety data sheet, use that manufacturer file. Hazard text does not belong in the sales description. For a pack size, a grade or a certificate that is not on the page, send a quote request or contact the team.
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The seven catalogues are Labware, Lab chemicals, Lab instruments, Lab consumables, Chromatography equipment, Lab safety equipment and Life Science & Microbiology.