Description
souris anti-tag DDDDK, monoclonal, AP1501, après dissolution dans 100 µl de PBS, 250 µg/ml, purifié par affinité (fabriqué par PROGEN), Densité (D) ~1 g/cm³, Point d'ébullition (pb) 100 °C, Température de stockage +4 °C
Anticorps primaires spécifiques qui reconnaissent les tags protéiques les plus couramment utilisés. Les anticorps peuvent être utilisés de manière optimale dans des études de biologie cellulaire ou tissulaire, des essais d'interaction protéine-protéine, des modifications post-traductionnelles, des études de dynamique d'expression ou de localisation, jusqu'à la production de kits analytiques à l'échelle du laboratoire ou à grande échelle.
- Disponible pour les tags protéiques les plus couramment utilisés (His, DDDDK, GST, HA, myc, GFP & Strep)
- Haute affinité pour le tag protéique respectif
- Détection robuste et hautement spécifique de la protéine de fusion
- Non conjugué
- Convient par exemple pour le western blot, l'immunoprécipitation, l'ELISA et l'immunofluorescence
L'anticorps monoclonal AP1501 reconnaît la séquence d'acides aminés DDDDK (Flag). Le tag DDDDK est couramment ajouté aux protéines recombinantes et peut être utilisé pour la détection ou la purification de la protéine taggée.
Immunocytochimie (ICC) : dépend de l'essaiImmunoprécipitation (IP) : dépend de l'essaiWestern Blot (WB) : 1:50 000-1:100 000 (0,005-0,0025 µg/ml)
| Application | ICC/IF IP WB |
| Espèce hôte | souris |
| Clonalité | monoclonal |
| Purification | Chromatographie d'affinité |
| Conjugué | onconjugé |
| Isotype | IgG2b |
| Clone | AP1501 |
| Cible | Tag DDDDK (FLAG) |
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Questions about souris anti-DDDDK-tag, monoclonal, AP1501, après dissolution dans 100 µl de PBS, 100 µl
What is this article?
souris anti-DDDDK-tag, monoclonal, AP1501, après dissolution dans 100 µl de PBS, 100 µl is the article on this page. Luminix Health distributes PROGEN for laboratory use. It sits in Primary antibodies, inside Antibodies & ELISA kits, in Microbiologie.
A primary antibody binds the target. Host species, clonality, clone name if monoclonal, the antigen, and the applications it was shown in, such as Western blot or immunohistochemistry, are the specification. A pretty band in the wrong application is not a promise.
An antibody binds one target. A primary antibody binds the molecule of interest. A secondary antibody binds the primary and usually carries the signal, an enzyme or a dye. An ELISA kit is that binding arranged in a well so the amount of target becomes a colour. The host species, the clone, the antigen and the stated application are the specification. A name alone is not. The literature is on PubMed. Sequences of the targets are on NCBI Nucleotide. Reference material is catalogued by ATCC.
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