Description
Ethidium bromide solut. 0.025 %, bottle, aqueous solution (250 µg/ml), ready-to-use, Density (D) 1 g/cm³, Boiling point (bp) ~100 °C
In spite of newly developed fluorescent staining reagents ethidium bromide remains the standard staining technic for nucleic acid in electrophoresis. Ethidium bromide is a carcinogenic substance which shouldn’t come in contact with skin. In order to minimise this danger we offer ready-to-use ethidium bromide solutions in dropper bottles for easy handling and maximum safety.
Excitation maxima (unbound): 210 nm, 285 nm, 470 nmExcitation maxima (bound to DNA): 330 nm, 500 nm. Excitation possible via DNA at 254 nmEmission maximum: 605 nm.
Ethidium bromide is a strong mutagenic and toxic substance. For your own safety, always wear gloves and respiratory protection to prevent contamination when working with this substance. After use, dispose of solutions correctly. They may not be discarded into the environment.The following procedure is recommended (acc. to INSERM, Dossier prevention, Number 2, 2nd Ed., September 88):1. Collect and dispose of as hazardous waste.2. Break down EtBr with potassium permanganate and HCI (P. Quillardet, M. Hofnung, Trends Genet. 4,89 [1988]).
Two drops will suffice for staining a 50 ml agarose gel. Add directly to melted agarose.
| Max. excitation (DNA-bound) | 330 nm500 nm |
| Max. excitation (unbound) | 210 nm 285 nm 470 nm |
| Emissions maximum | 605 nm |
| Use | Addition to melted agarose |