Description
Oxidation reagent, for DNA-Synthesis, for Expedite89xx and Polygen®, Density (D) 0,8 g/cm³, Flash point (flp) -21 °C, Boiling point (bp) 65 °C, UN-Nr. 1993
0,05 M Iodine
The phosphite triester linkage formed during oligonucleotide synthesis is of limited stability and has to stabilised. Treatment of the support-bound material with iodine and water in the presence of a weak base oxidizes the phosphite triester into a tetracoordinated phosphate triester, a protected precursor of the naturally occurring phosphate diester internucleosidic linkage.
Technical Information
| Instructions for use, addition | Suitable for Expedite™89xx and Polygen® |